Chemical cross-linking for protein-protein interaction studies

Chemical Cross

Chemical cross-linking is becoming a valuable tool for the high-order structure determination of proteins and protein complexes Cross-linking methodology is able to provide low-resolution structures when at least something is known already about the proteins under investigation

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Reliable Identification of Cross

We describe the use of the 13C-labeled artificial amino acid p-benzoyl-L-phenylalanine (Bpa) to improve the reliability of cross-linked product identification Our strategy is exemplified for two protein–peptide complexes These studies indicate that in many cases the identification of a cross-link without additional stable isotope labeling would result in an ambiguous assignment of cross

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Protein–Protein Interactions in High Moisture‐Extruded

7/10/2007Feng Liang Chen Yi Min Wei Bo Zhang Chemical cross-linking and molecular aggregation of soybean protein during extrusion cooking at low and high moisture content LWT - Food Science and Technology 10 1016/j lwt 2010 12 008 44 4 (957-962) (2011)

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Visualizing the Ensemble Structures of Protein Complexes

Visualizing the Ensemble Structures of Protein Complexes Using Chemical Cross-Linking Coupled with Mass Spectrometry Biophysics Reports Dec 2015 Zhou Gong Yue-He Ding Xu Dong Na Liu E Erquan Zhang Meng-Qiu Dong Chun Tang

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Protein‐Protein Interactions Identified by Pull‐Down

B Stynen H Tournu J Tavernier P Van Dijck Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System Microbiology and Molecular Biology Reviews 10 1128/MMBR 05021-11 76 2 (331-382) (2012)

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Protein‐Protein Interactions Identified by Pull‐Down

B Stynen H Tournu J Tavernier P Van Dijck Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System Microbiology and Molecular Biology Reviews 10 1128/MMBR 05021-11 76 2 (331-382) (2012)

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Stitching the synapse: Cross

The identification of 1036 protein pairs cross-linked enabled us to construct an XL-based protein-protein interaction map of the synapse The PPI network of individual subcellular fractions and brain areas are shown in figs S3 to S6 and the combined network is shown in Fig 3A (from dataset 1)

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Bridging structural biology and genomics: assessing

quality of these interaction datasets by either integrating additional annotation (e g synexpression among interacting proteins) or through comparison with reference interaction sets [8–11] The conclusions from these studies were that the interaction datasets contain false positives and are missing many true protein interactions

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Crystallography and protein–protein interactions

ation on protein–protein complexes and it yields the most detailed structural information about the interaction How-ever there is a major issue when using MX to study protein– protein interactions: a crystal structure does not define a unique protein–protein interface A crystal is a regular 3D

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Proteins cross linking reagents Medical search FAQ

Proteins cross linking reagents English English Espaol Portugus Franais Italiano Svenska Deutsch Home page Questions and answers Statistics Advertise with us Contact Anatomy 10 Cell Line Cell Membrane Cells Cultured Immune Sera Blood-Brain Barrier Erythrocytes Chromosomes Plant Liver Brain Cell Line Tumor

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Diversity in Genetic In Vivo Methods for Protein

The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction methods and undisputedly gave rise to a palette of ingenious techniques that are constantly pushing further the limits of the original method Sensitivity and selectivity have improved because of various technical tricks and experimental designs Here we present an exhaustive overview of the genetic

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1601358

crosslinking For protein:protein interaction studies the proximity between reactive groups is difficult to predict Usually a crosslinker with a short (4-8 ) spacer arm is used first and the degree of crosslinking determined A crosslinker with a longer spacer arm may then be used to optimize crosslinking efficiency

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Isotope

devise strategies that enable the mapping of protein-protein interaction surfaces even at low resolution We and others have recently shown that valuable spatial restraints can be obtained from chemical cross-linking of protein assemblies through use of tandem mass spectrometry to identify the specific cross-linked amino acid participants

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Isotope

devise strategies that enable the mapping of protein-protein interaction surfaces even at low resolution We and others have recently shown that valuable spatial restraints can be obtained from chemical cross-linking of protein assemblies through use of tandem mass spectrometry to identify the specific cross-linked amino acid participants

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Identification and Mapping of Protein−Protein Interactions

7/1/2005Chemical Cross-Linking for Protein–Protein Interaction Studies 2009 283-293 DOI: 10 1007/978-1-59745-493-3_17 Daniel J Cipriano Stanley D Dunn Tethering polypeptides through bifunctional PEG cross-linking agents to probe protein function: Application to ATP synthase

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Optimized Cross

Optimized Cross-Linking Mass Spectrometry for in Situ Interaction Proteomics Zheng Ser † ‡ Paolo Cifani † and Alex Kentsis* † ‡ ∥ †Molecular Pharmacology Program Sloan Kettering Institute ‡Tri-Institutional Ph D Program in Chemical Biology and Department of Pediatrics Memorial Sloan Kettering Cancer Center New York New York 10065 United States

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Scope limitations and mechanistic aspects of the photo

Background: Chemical cross-linking is a valuable tool with which to study protein–protein interactions Recently a new kind of cross-linking reaction was developed in which the photolysis of associated proteins with visible light in the presence of ammonium persulfate and tris(2 2′-bipyridyl)ruthenium(II) dication or palladium(II) porphyrins results in rapid and efficient covalent

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Protein

Recent technical advancements of the chemical cross-linking methods achieved in a number of labs have allowed this technique to be extended to complex systems Successful applications of chemical cross-linking to studies of intact virus particles cell lysates and even intact bacterial and human cells suggest that in the future cross-linking methods may provide a majority of structural and

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A multipronged approach unravels unprecedented protein

We carried out multiple physico-chemical studies including fluorescence hydrogen– deuterium exchange MS (HDX-MS) and chemical cross-linking MS (CL-MS) Our fluorescence studies suggested a strong interaction for the hE1o– hE2o subcomplex but a much weaker interaction in the hE1o– hE3 subcomplex and failed to identify any interaction

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Crosslinking Protein Interaction Analysis

Yakovlev AA (2009) Crosslinkers and their utilization for studies of intermolecular interactions Neurochem J 3:139–144 Yang L et al (2010) A photocleavable and mass spectrometry identifiable cross-linker for protein interaction studies Anal Chem 82:3556–3566

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[1606 04247] Characterization of protein complexes using

This is a useful methodology for studying protein networks using mass spectrometry Included are a list of GFP non-specific interactions and detailed methods for protein-protein interaction studies: Subjects: Molecular Networks (q-bio MN) Biomolecules (q-bio BM) Cite as: arXiv:1606 04247 [q-bio MN] (or arXiv:1606 04247v1 [q-bio MN] for this

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